FIG. 7.

The effect of NPPB on (A) the phosphorylation of Erk1/2, p38, and JNK/SAPK MAP kinases, (B) iNOS protein expression, and (C) NO production in LPS-stimulated RAW cells. RAW cells were pretreated with NPPB (100 μM) for 30 min, followed by treatment with LPS (1 μg/ml) for 30 min. The cells were lysed, and the lysates were analyzed by immunoblotting, using (A) the antibodies to phospho-Erk1/2, phospho-p38, phospho-JNK/SAPK, Erk, p38, or JNK/SAPK or (B) iNOS. (C) RAW cells were pretreated with NPPB (100 μM) for 30 min. NPPB pretreated and untreated RAW cells were incubated with LPS (1 μg/ml) for 24 h.