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. 2022 Nov 12;1(5):pgac231. doi: 10.1093/pnasnexus/pgac231

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© The Author(s) 2022. Published by Oxford University Press on behalf of the National Academy of Sciences.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Switching frequency of VIR-O mutants. Panel A: Individual TTTR mutants with the relevant mutation shown below each strain. Panel B: Switching frequency for the triple (VIR-O.3KO) and quadruple TTTR (VIR-O.4KO) mutants along with VIR-O.4KO complemented with ABUW_3353 (3353C). Each value represents the switching frequency from VIR-O to the translucent variant and was determined from the average of three to six colonies after 24 hours of growth on O.5X LB agar plates with SDs shown. **P-value = 0.0019 (Welch’s ANOVA)