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. 2022 Nov 12;1(5):pgac231. doi: 10.1093/pnasnexus/pgac231

Fig. 4.

Fig. 4.

Expression of TTTRs in translucent subpopulations. Panel A: Expression of ABUW_1645-lacZ,ABUW_2818-lacZ, and ABUW_1959-lacZ fusions in VIR-O and AV-T.LS cells. Values represent the averages duplicate samples from two biological replicates with SDs. *P-value = 0.01, ***P-value = 0.0008, ****P-value = 0.0001 determined by unpaired t-test. Panel B: Frequency of independent translucent variant cells expressing each TTTR-lacZ fusion. For AB5075, numbers are based on 150 independent cells. For AB0057, 100 independent cells were used. Panel C: Expression of ABUW_1645-gfp, ABUW_2818-mCherry, and ABUW_1959-mTAG BFP2 in cells of VIR-O colony grown on a 0.5X LB agar plate and taken at 12 hours of growth. Photos were taken using a Neo 5.5 scientific CMOS camera (Andor) via an inverted microscope (Olympus IX83P2Z). Cells were imaged using phase contrast as well as GFP, Texas Red, and DAPI filters to capture ABUW_1645-gfp, ABUW_2818-mCherry, and ABUW_1959-mTAG BFP2 fluorescence, respectively.