Table 1.
Phenotypes of VIR-O cells overexpressing TetR regulators
| TetR overexpressed | Opacitya | QS signal secretionb | Motilityc |
|---|---|---|---|
| ABUW_1645 | Translucent | – | – |
| ABUW_2818 | Translucent | – | + |
| ABUW_1959 | Translucent | – | – |
| ABUW_3353 | Translucent | – | – |
| ABUW_2596 | Translucent | – | – |
| ABUW_1498 | Translucent | – | – |
| ABUW_3194 | Translucent | – | + |
| ABUW_1163 | Translucent | – | – |
| ABUW_0222 | Translucent | – | – |
| ABUW_2629 | Translucent | – | + |
| ABUW_1912 | Translucent | – | – |
a
Determined on 0.5× agar plates using oblique lighting.
b
Determined by plating on a soft agar lawn containing an A. tumefaciens traG-lacZ biosensor for detection of 3-OH-C12-HSL. A (–) indicates greater than 70% reduction in halo size relative to cells with pWH1266 vector alone.
c
Determined on 0.35% Eiken agar plates. A (–) indicates motility that was at least 30% less than with pWH1266 vector alone based on three biological replicates.