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. 2022 Oct 26;1(5):pgac241. doi: 10.1093/pnasnexus/pgac241

Fig. 2.

Fig. 2.

Effects of deleting ncRNAs on adjacent protein-coding genes. (A) Quantification of APM1 and THI21 transcript level in the SUT390 deletion strain compared to the wild-type strain. N = 6 for SUT390, N = 2 for wild-type. (B) Quantification of YPR015C transcript level in the SUT418 deletion strain compared to the wild-type strain. N = 5 for SUT418△, N = 5 for wild-type. (C) Quantification of PIL1 transcript level in the SUT125 deletion strain compared to the wild-type strain. N = 6 for SUT125△, N = 2 for wild-type. (D) Organization of SUT390, SUT418, SUT125 and the respective neighboring protein-coding genes in the yeast genome. Primer pairs indicate the region of each gene examined by RT-qPCR (purple). Unpaired parametric t test was used. *** P = 0.0009 and *P = 0.0378.