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. 2022 Nov 4;1(5):pgac248. doi: 10.1093/pnasnexus/pgac248

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© The Author(s) 2022. Published by Oxford University Press on behalf of National Academy of Sciences.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1. — NXT-2 immunization generates antifungal cross-reactive antibodies. (A) The NXT-2 sequence was generated to a consensus sequence with homology to KEX1 regions of Pneumocystis (PC.KEX1),Aspergillus (AF.KEX1),Candida (CA.KEX1), and Cryptococcus (CN.KEX1), by amino acid type (Clustal Omega). (B) Percent identity of each KEX1 variant against NXT-2 and the western blots developed using plasma from NHPs immunized with NXT-2a + alum or PBS + alum. Antibodies to NXT-2a bind to NXT-2 (Blot 1, lane 2), AF.KEX1 (Blot 1, lane 3), PC.KEX1 (Blot 1, lane 4), and CA.KEX1 (Blot 1, lane 5). Antibodies to NXT-2a further bind to Histoplasma capsulatum KEX1 (HC.KEX2; Blot 2, lane 3), Coccidioides immitis KEX1 (CI.KEX1; Blot 1, lane 4), Mucor circinelloides KEX1 (MC.KEX1; Blot 1, lane 5), Candida auris KEX1 (CAu.KEX1; Blot 1, lane 3), and C. neoformans KEX1 (CN.KEX1; Blot 1, lane 4). (C) The correlation of % identity and the % western blot saturation quantified by ImageJ relative to NXT-2 (P = 0.0009).