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. 2022 Nov 4;1(5):pgac248. doi: 10.1093/pnasnexus/pgac248

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© The Author(s) 2022. Published by Oxford University Press on behalf of National Academy of Sciences.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — NXT-2 vaccination reduces invasive pulmonary aspergillosis in a model of solid organ transplantation. (A) Aspergillosis challenge study design. Vaccination is indicated with arrows. BALB/c mice were immunized with NXT-2 + TiterMax, AF.KEX1 + TiterMax, or PBS + TiterMax 28 days prior to immunosuppression with hydrocortisone and tacrolimus. (B) NXT-2 plasma IgG reciprocal endpoint titers (RET) in mice immunized with NXT-2 + TiterMax or PBS + TiterMax. Presented RET is against the antigen with which mice were vaccinated. SHAM data is an average of anti-NXT-2 and anti-AF.KEX1 RET. (C) Survival curve of NXT-2- and AF.KEX1-immunized animals, compared with SHAM. NXT-2- and AF.KEX1-immunized animals were significantly protected from developing aspergillosis, compared with mock-immunized controls (*P = 0.0118; *P = 0.0172, by Mantel–Cox test). (D) Quantification of fungal burden in a solid organ transplant model by %GMS + area. (E) Percent weight loss 10 days from Aspergillus challenge with significant differences between NXT-2-vaccinated and Sham at 7-, 8-, and 10-days post infection. Data represents the mean ± SD. Differences in weight loss were analyzed by repeated measures mixed modeling. (F) GMS and H&E staining of lung tissue following SHAM or (G) NXT-2 immunization and Aspergillus challenge (10x magnification). ** < 0.01, ^***< 0.001.