Fig. 2.

(a) Schematic diagram of the experimental setup. Aliquots of S. marcescens were placed on PAA substrates that were 0.8 mm in height. The substrates were maintained at 37°C in a humid stage top incubator. (b) Visualization of the growing biofilm boundary overlaid on a sample image of the biofilm. Images were acquired at 10-minute increments, and boundaries shown here are displayed at 20-minute increments. (c) Color image of full bacterial colony after 15 hours of growth. Scale bars, 1 mm.