Figure 1.
Interferon -γ contributes to CD8T-mediated salt-sensitive hypertension. (A) Radiotelemetry recording of systolic BP in recipient wild-type (C57/B6) mice (blue) and IFN-γ knockout (B6 background) mice (red) after adoptive transfer of 1×107 CD8Ts freshly isolated from deoxycorticosterone acetate+salt-treated IFN-γ knockout or wild-type mice. All mice were purchased from the Jackson Laboratory. Data were recorded every 15 minutes and averaged to six time points a day; n=5–6 mice per group. Statistical analysis was performed by two-way ANOVA. Significance for interaction effect: P<0.001; time effect: P<0.001; and strain recipient: P=0.02. (B) At the end point of BP recording in (A), immunohistochemistry staining (DAB) of kidney sections was performed using CD3 specific monoclonal antibody after the protocol we have published previously (42,43). In the quantification bar graph, each dot shown is the mean of four images taken per sectioned kidney. **P<0.05.