FIG. 5.
Dixon plot for the determination of the inhibition constant (Ki) of either histatin 5 (A) or leupetin (B) against Arg-gingipain. Enzyme assays were carried out with 3.3 nM gingipain in the presence of increasing concentrations of histatin 5 at two different concentrations of BAPNA (60 and 106 μM). The reciprocals of velocity were plotted against the histatin 5 concentration, and the Ki value was obtained from the intercepts of two lines at two concentrations of substrate.