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. 2001 Mar;69(3):1402–1408. doi: 10.1128/IAI.69.3.1402-1408.2001

FIG. 5.

FIG. 5

Dixon plot for the determination of the inhibition constant (Ki) of either histatin 5 (A) or leupetin (B) against Arg-gingipain. Enzyme assays were carried out with 3.3 nM gingipain in the presence of increasing concentrations of histatin 5 at two different concentrations of BAPNA (60 and 106 μM). The reciprocals of velocity were plotted against the histatin 5 concentration, and the Ki value was obtained from the intercepts of two lines at two concentrations of substrate.