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. 2022 Dec 24;4(1):101962. doi: 10.1016/j.xpro.2022.101962
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Anti-FLAG (1:10,000 dilution) Sigma-Aldrich Cat# A8592
Anti-penta-His (1:10,000 dilution) Qiagen Cat# 34460

Bacterial and virus strains

Escherichia coli BL21(DE3) (genotype: E. coli B dcm ompT hsdS(rB-mB-) gal with λDE3 prophage) Novagen Cat# 69450

Chemicals, peptides, and recombinant proteins

Acteone Sigma-Aldrich Cat# 179124-4L-PB
Bromophenol blue Bio-Rad Cat# 161-0404
Chloramphenicol (Cm) Roche Cat# 10 634 433 001
CuSO4·5 H2O Sigma-Aldrich Cat# C7631
Dithiothreitol (DTT) Sigma-Aldrich Cat# D0632-25G
Dimethyl sulfoxide (DMSO) J.T. Baker Cat# 9224-01
Ethylenediaminetetraacetic acid (EDTA) Invitrogen Cat# 15576-028
Methanol Sigma-Aldrich Cat# 179337-4L-PB
Neocuproine Sigma-Aldrich Cat# N1501
N-ethylmaleimide (NEM) Sigma-Aldrich Cat# E3876
1,10-Phenanthroline monohydrate (2-phenanthroline) Sigma-Aldrich Cat# P9375
Trichloroacetic acid (TCA) J.T.Baker Cat# 0414-01
Tris base Invitrogen Cat# 15504-020
Sodium dodecyl sulfate (SDS) Bio-Rad Cat# 161-0302
Western Lightning Plus, Chemiluminescent Substrate PerkinElmer Cat# NEL105001EA

Critical commercial assays

QuikChange II Site-Directed Mutagenesis Kit Agilent Cat# 200523

Software and algorithms

Image Lab Bio-Rad Version 5.2.1

Other

Ambient temperature (20°C–25°C) microcentrifuge for 1.5–2.0 mL tubes N/A N/A
Cooled (4°C–12°C) microcentrifuge for 1.5–2.0 mL tubes N/A N/A
Incubator shaker N/A N/A
Klett-Summerson photoelectric colorimeter or spectrophotometer N/A N/A
Orbital shaker N/A N/A
Vortexer N/A N/A
Water bath or heat block N/A N/A