| Antibodies |
|
| Anti-FLAG (1:10,000 dilution) |
Sigma-Aldrich |
Cat# A8592 |
| Anti-penta-His (1:10,000 dilution) |
Qiagen |
Cat# 34460 |
|
| Bacterial and virus strains |
|
|
Escherichia coli BL21(DE3) (genotype: E. coli B dcm ompT hsdS(rB-mB-) gal with λDE3 prophage) |
Novagen |
Cat# 69450 |
|
| Chemicals, peptides, and recombinant proteins |
|
| Acteone |
Sigma-Aldrich |
Cat# 179124-4L-PB |
| Bromophenol blue |
Bio-Rad |
Cat# 161-0404 |
| Chloramphenicol (Cm) |
Roche |
Cat# 10 634 433 001 |
| CuSO4·5 H2O |
Sigma-Aldrich |
Cat# C7631 |
| Dithiothreitol (DTT) |
Sigma-Aldrich |
Cat# D0632-25G |
| Dimethyl sulfoxide (DMSO) |
J.T. Baker |
Cat# 9224-01 |
| Ethylenediaminetetraacetic acid (EDTA) |
Invitrogen |
Cat# 15576-028 |
| Methanol |
Sigma-Aldrich |
Cat# 179337-4L-PB |
| Neocuproine |
Sigma-Aldrich |
Cat# N1501 |
|
N-ethylmaleimide (NEM) |
Sigma-Aldrich |
Cat# E3876 |
| 1,10-Phenanthroline monohydrate (2-phenanthroline) |
Sigma-Aldrich |
Cat# P9375 |
| Trichloroacetic acid (TCA) |
J.T.Baker |
Cat# 0414-01 |
| Tris base |
Invitrogen |
Cat# 15504-020 |
| Sodium dodecyl sulfate (SDS) |
Bio-Rad |
Cat# 161-0302 |
| Western Lightning Plus, Chemiluminescent Substrate |
PerkinElmer |
Cat# NEL105001EA |
|
| Critical commercial assays |
|
| QuikChange II Site-Directed Mutagenesis Kit |
Agilent |
Cat# 200523 |
|
| Software and algorithms |
|
| Image Lab |
Bio-Rad |
Version 5.2.1 |
|
| Other |
|
| Ambient temperature (20°C–25°C) microcentrifuge for 1.5–2.0 mL tubes |
N/A |
N/A |
| Cooled (4°C–12°C) microcentrifuge for 1.5–2.0 mL tubes |
N/A |
N/A |
| Incubator shaker |
N/A |
N/A |
| Klett-Summerson photoelectric colorimeter or spectrophotometer |
N/A |
N/A |
| Orbital shaker |
N/A |
N/A |
| Vortexer |
N/A |
N/A |
| Water bath or heat block |
N/A |
N/A |