Figure 5.

Demethylase-deficient lsd1 rescues hematopoietic transcription factor gene expression

(A and B) Single-cell zebrafish embryos from a kdm1a(23095) heterozygous incross were injected with zebrafish zlsd1-K661A. WISH was then performed at 24 hpf for gata1 (A) and gfi1ab (B). Representative images are shown with the number of animals with similar staining patterns for the indicated genotype shown in the upper right corner. The lower right corner indicates the mRNA injection conditions. Uninjected mutant embryos are shown in the middle column for comparison. Embryos were imaged, blindly scored by expression and then genotyped by HRMA. Carons indicate ICM expression; closed arrowheads indicate posterior blood island/caudal hematopoietic tissue; narrow triangles indicate inner-hair-cell expression. Scale bar, 200 μm.