Antibodies |
|
Anti-Caspase 3 |
BD Bioscience |
RRID: AB_397274; Cat#559565 |
Donkey Anti-Rabbit Alexa Fluor 568 |
Life Technologies |
RRID: AB_2534017; Cat#A10042 |
Anti-Digoxigenin |
Sigma Aldrich |
RRID: AB_2734716; Cat#11093274910 |
Anti-Flag M2 |
Sigma Aldrich |
RRID: AB_259529; Cat#F3165 |
HRP-Donkey Anti-Mouse IgG |
Jackson Immunoresearch |
RRID: AB_2340770; Cat#715-035-150 |
Anti-Phospho-Histone H3 |
Cell Signaling Technology |
RRID: AB_331535; Cat#9701 |
|
Chemicals, peptides, and recombinant proteins |
|
SNAG peptide |
GenScript |
N/A |
Proteinase K |
Sigma Aldrich |
Cat#RPROTK-RO |
Ambion SP6 RNA Polymerase |
Invitrogen |
Cat#AM2071 |
Ambion T7 RNA Polymerase |
Invitrogen |
Cat#AM2082 |
Ribonucleic Acid Type VI |
Sigma Aldrich |
Cat#R6625 |
Heparin |
Sigma Aldrich |
Cat#H3393 |
Maleic Acid |
Sigma Aldrich |
Cat#M0375 |
Blocking Reagent |
Roche |
Cat#11096176001 |
Fetal Bovine Serum, Heat Inactivated |
Gibco |
Cat#10438026 |
RNAlater |
Qiagen |
Cat#76104 |
Phusion High-Fidelity DNA Polymerase |
NEB |
Cat#M0530S |
Protease Inhibitor Cocktail |
Sigma Aldrich |
Cat#P8340 |
gfi1b sgRNA |
This paper |
N/A |
|
Critical commercial assays |
|
OneStep RT-PCR |
Qiagen |
Cat#210210 |
QuikChange II Site-Directed Mutagenesis Kit |
Agilent |
Cat#200523 |
QIAquick Gel Extraction Kit |
Qiagen |
Cat#28704 |
pGEM-T-Easy Vector Systems |
Promega |
Cat#A1360 |
LightScanner Master Mix |
BioFire Defense |
Cat#HRLS-ASY-0003 |
mMessage mMachine SP6 Transcription Kit |
Invitrogen |
Cat#AM1340 |
NucAway Spin Columns |
Thermo Fisher |
Cat#AM10070 |
DIG RNA Labeling Mix |
Sigma Aldrich |
Cat#11277073910 |
BCIP/NBT Substrate Kit |
Vector Laboratories |
Cat#SK5400 |
PureLink RNA MicroKit |
Invitrogen |
Cat#12183016 |
SuperSignal West Femto Maximum Sensitivity Substrate |
Thermo Fisher |
Cat#34095 |
Cas9 |
IDT |
Cat#1081058 |
HiScribe T7 Quick High Yield RNA Synthesis RNA Kit |
NEB |
Cat#E2050S |
RNeasy Plus Mini Kit |
Qiagen |
Cat#74134 |
Protease inhibitor cocktail |
Sigma Aldrich |
Cat#P8340 |
PVDF membrane |
Millipore |
Cat#IPVH00010 |
SuperSignal West Femto Maximum Sensitivity Substrate |
Thermo Fisher |
Cat#34095 |
Autoradiography film |
Genesee Scientific |
Cat#30-507 |
|
Deposited data |
|
Raw data |
This paper |
GEO: GSE198476
|
|
Experimental models: Organisms/strains |
|
Zebrafish: kdm1a 23095
|
|
ZFIN: ZDB-ALT-131217-16908 |
Zebrafish: kdm1a 36433
|
|
ZFIN: ZDB-ALT-161003-15414 |
Zebrafish: gfi1aa 16850
|
|
ZFIN: ZDB-ALT-131217-13025 |
Zebrafish: gfi1ab 40706
|
|
ZFIN: ZDB-ALT-160601-6870 |
Zebrafish: plrg1 hi3174aTg
|
|
ZFIN: ZDB-ALT-040826-6 |
Zebrafish: gfi1b Δ17
|
This paper |
N/A |
|
Oligonucleotides |
|
Primers for genotyping, see Table S2
|
This paper |
N/A |
Primers for cDNA amplification, see Table S2
|
This paper |
N/A |
Primers for site directed mutagenesis, see Table S2
|
This paper |
N/A |
|
Recombinant DNA |
|
pUC57-zlsd1-3XFLAG |
This paper |
N/A |
pUC57-zlsd1-K661A |
This paper |
N/A |
pUC57-zlsd1-ΔQ632-3XFLAG |
This paper |
N/A |
pUC57-zlsd1-D555K-3XFLAG |
This paper |
N/A |
pUC57-zlsd1-A539E-3XFLAG |
This paper |
N/A |
pUC57-zlsd1-ΔTower-3XFLAG |
This paper |
N/A |
pUC57-zlsd1-ΔAOLC |
This paper |
N/A |
pUC57-hLsd1-3XFLAG |
This paper |
N/A |
pUC57-hLsd1-ΔAOLC |
This paper |
N/A |
pGEM-T-Easy-gfi1b |
This paper |
N/A |
pGEM-T-Easy-gfi1aa |
This paper |
N/A |
pGEM-T-Easy-gfi1ab |
This paper |
N/A |
pGEM-T-Easy-fli1a |
This paper |
N/A |
pBluescript-gata1 |
Detrich et al.76
|
N/A |
pUC57-etv2 |
Sumanas et al.77
|
N/A |
pBK-snail1a |
Thisse et al.78
|
N/A |
pBK-snail1b |
Thisse et al.79
|
N/A |
pBS-IISK-snai2 |
Thisse et al.80
|
ZFIN: ZDB-EST-030328-40 |
|
Software and algorithms |
|
ImageJ |
|
|
LightScanner Call-IT |
Idaho Technology Inc. |
|
Adobe Illustrator |
Adobe |
|
Adobe Photoshop |
Adobe |
|
Integrated Genomics Viewer |
Broad Institute |
|
RStudio |
RStudio |
|
Pymol |
Schrodinger Inc. |
|
GraphPad Prism |
Dotmatics |
|