Fig. 5.

In vitro antitumor efficacy of M_ICG-Pt. (a) Live/dead staining of B16F10 cells after incubation with different microparticles for 24 h. Scale bar, 100 μm. (b, c) Apoptosis analysis (b) by flow cytometry and corresponding apoptosis rates (c) of B16F10 cells after incubation with different microparticles for 24 h. (d) CCK8 assay of B16F10 cells after incubation with different microparticles for 24 h (−) indicated without NIR irradiation, and (+) indicated with NIR irradiation (808-nm, 0.6 W cm⁻², 5 min). All the cell experiments had three independent replicates (n = 3). Data are presented as the mean ± SD. n.s.: no significance, *p < 0.05, **p < 0.01, ***p < 0.001.