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Immunohistochemistry and immunofluorescence analyses. (a) H&E, TUNEL, and HIF-1α staining images of tumor tissues after various treatments. Scale bar, 50 μm. (b–d) Quantification of the H&E (b), TUNEL (c), and HIF-1α (d) staining assays. (−) indicated without NIR irradiation, and (+) indicated with NIR irradiation. Each experiment group has 3 mice (n = 3). Data are presented as the mean ± SD. n.s.: no significance, *p < 0.05, **p < 0.01, ***p < 0.001.
