Figure 2.

Comparative OCT B-scans and corresponding A-line profiles from both strains under light/dark conditions. (A) Representative OCT of inferior outer retina for light-dark wildtype and Gnat1^rd17 mice. Green arrow: direction of A-line profiles in B). (B) Average superior A-line profiles from images shown in A) for 60 minute light-adapted wildtype (n = 5 per condition; top) and a Gnat1^rd17 (n = 5 per condition; bottom) mice; zoomed-in region of the dark wildtype data is shown in the black box to illustrate the smaller HB magnitude. The ISez (pink), ELM-RPE (blue), and HB (red) indexes are shown. The light-adapted Gnat1^rd17 mouse has a visably rounder ISez, thinning of its ELM-RPE region, and no discernable HB signal compared to the light-adapted wildtype mouse; together these biomarkers raise the possibility of a higher energy demand condition in Gnat1^rd17 rod mitochondria; other interpretations are possible (see Discussion). No upsampling/zero-filling is used for the A-line profiles.