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. 2022 Nov 23;29(1):365–377. doi: 10.1111/cns.14011

FIGURE 5.

FIGURE 5

Altered expression in the MAM fraction and PTPIP51 phosphorylation in the presence of TAT. (A) Isolation of MAMs (white ring). (B) Western blot analysis of the expression of PTPIP51 and VAPB in different fractions of control SH‐SY5Y cells and those treated with Tat. (C) Representative images of PLA for tyrosine phosphorylated PTPIP51 (pPTPIP51) in LUHMES cells in the absence and presence of Tat. Red dots indicate phospho‐tyrosine residues. DAPI staining indicates individual nuclei. (D) Quantification of the PLA for pPTPIP51 represented as a bar graph. (E) Representative images of PLA for PTPIP51 and VAPB in LUHMES cells treated with kinase inhibitors (gefitinib, dasatinib, and RpcAMPs) for 4 h in the absence and presence of Tat. Red dots indicate individual interactions. DAPI staining indicates individual nuclei. (F) Quantification of the number of PTPIP51 and VAPB interactions is represented as a bar graph. N.S = not significant, *p < 0.05. Error bars are shown as S.E.M.