Figure 5.
Behavioral effects in the gustatory and olfactory system
(A) Summary of percentage of different outcomes on performing proboscis extension response assay on labellar stimulation of female CS flies to 100mM sucrose alone as well as mixed with indicated percentage of NH4OH (left panel). Summary of percentage of different outcomes on proboscis extension response assays on labellar stimulation of female CS flies to 100 mM sucrose alone (pH 6.8) as well as mixed with two different NaOH concentrations to attain the indicated pH (right panel). As indicated in numbers above each graph, percentage of flies showing full extension was indicated in purple bar and ascribed a value of +1, percentage with a half-extension or extension with immediate retraction was ascribed a red bar and a value of +0.5, and percentage with no extension in blue with a value of 0 (blue bar). n = 14 flies in both cases.
(B) Representative image (left) and mean count of maximum number of wildtype Aedes aegypti females landed on nylon mesh with human odor in a 9 min period, after a 30-s exposure to either a water control or 3.3% ammonia (right). Mated females, 10–12 days old. Maximum mosquito count frame was determined from the 90 frames during the 9-min video. n = 5 trials of 19–21 mosquitos for each condition. T test. Error bars indicate SEM ∗∗∗p < 0.001.
(C) Mean count represented over different time-points from the same experiment as above. T test. Error bars indicate SEM ∗p < 0.05.