Figure 2.

FAPs are the predominant cells expressing extracellular matrix (ECM) genes in both WT and mdx^5cv quadriceps and diaphragm

(A) Dot plot showing the expression of collagen and fibronectin genes by different cell types in different muscles. The dot color intensity reflects the average gene expression level, and the dot size represents the percentage of the cells expressing the gene.

(B) Dot plot showing the expression of proteoglycan genes.

(C) qRT-PCR showing and comparing Col1a1 and Col3a1, and Fn1 expression by FACS-sorted FAPs, FACS-sorted non-FAPs, and whole muscle of mdx^5cv quadriceps and diaphragm at 14 weeks of age. Data are represented as mean ± SEM Asterisks indicate significant differences (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ns: no significance, Kruskal-Wallis test followed by Dunn’s test for multiple comparisons). N = 5 mice/group.