REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti- a/b-tubulin	Cell Signaling Technology	Cat# 2148S; RRID: AB_2288042
Rabbit anti-collagen 1a1	Cell Signaling Technology	Cat# 72026S; RRID: AB_2904565
Rabbit anti-fibronectin	Cell Signaling Technology	Cat# 63779S
Rabbit anti-collagen III	Abcam	Cat# ab7778; RRID:AB_306066
IRDye 800CW anti-rabbit IgG	LI-COR Biosciences	Cat# 926-32211; RRID: AB_621843
PerCP-Cy5.5 Rat anti-mouse CD45	BD Biosciences	Cat# 550994; RRID:AB_394003
PE Rat anti-mouse CD45	BD Biosciences	Cat# 553081; RRID:AB_394611
APC Rat anti-mouse CD31	BD Biosciences	Cat# 551262; RRID:AB_398497
PE Rat anti-mouse CD31	BD Biosciences	Cat# 553373; RRID:AB_394819
PE-Cy7 Rat anti-mouse Ly6-A/E (Sca-1)	BD Biosciences	Cat# 558162; RRID:AB_647253
BV421 Rat anti-mouse CD140A (PDGFRa)	BD Biosciences	Cat# 562774; RRID:AB_2728781
PE Rat anti-mouse Integrin α7	R&D Systems	Cat# FAB3518P; RRID:AB_2249296
APC Rat IgG2a, κ Isotype Control	BD Biosciences	Cat# 551139; RRID: AB_10054921
PE Rat IgG2a, κ Isotype Control	BD Biosciences	Cat# 559317; RRID: AB_10050484
PE-Cy7 Rat IgG2a, κ Isotype Control	BD Biosciences	Cat# 552784; RRID: AB_394465
BV421 Rat IgG2a, k Isotype Control	BD Biosciences	Cat# 562602; RRID:AB_11153860
PerCP-Cy5.5 Rat IgG2b, κ Isotype Control	BD Biosciences	Cat# 550764; RRID: AB_393874
PE Rat IgG2b, k Isotype Control	BD Biosciences	Cat# 555848; RRID:AB_396171
Chemicals, peptides, and recombinant proteins
Mayer’s Hematoxylin solution	Millipore Sigma	Cat# MHS16
Eosin Y solution	Millipore Sigma	Cat# HT110116
4% paraformaldehyde solution	Thermo Scientific	Cat# J19943.K2
Poly Mount Xylene	Polysciences	Cat# 24716-120
Collagenase B	Roche Diagnostics	Cat# 11088831001
Dispase II	Roche Diagnostics	Cat# 04942078001
Fetal bovine serum	Corning	Cat# 35-011-CV
Bovine serum albumin	Millipore Sigma	Cat# A7030
Normal mouse serum	Millipore Sigma	Cat# S7273
Lympholyte-M solution	Cedarlane	Cat# CL5035
TRIzol reagent	Invitrogen	Cat# 15596026
RPMI 1640	Gibco	Cat# 11-875-093
RIPA buffer	Pierce Biotechnology	Cat# 89901
cOmplete (protease inhibitor)	Roche Diagnostics	Cat# 04.693.116.001
Bolt 4–12% Bis-Tris mini-gel	Invitrogen	Cat# NW04122
Immobilon PVDF membrane	Millipore	Cat#I SEQ00010
Acridine Orange and Propidium Iodide staining solution	Nexcelom	Cat# CS2-0106
Critical commercial assays
Vybrant™ DyeCycle™ Violet/SYTOX™ AADvanced™ Kit	Invitrogen	Cat# A35135
EdU Click 647 Kit + EdU	Baseclick GmbH	Cat# BCK647
Rneasy Micro Kit	Qiagen	Cat# 74004
Super-Script III Reverse Transcriptase Kit	Invitrogen	Cat# 18080051
PowerTrack™ SYBR Green Master Mix	Applied Biosystems	Cat# A46109
BCA protein assay Kit	Pierce Biotechnology	Cat# 23225
Single Cell 3′ Reagents Kits V3.1	10x Genomics	https://assets.ctfassets.net/an68im79xiti/1eX2FPdpeCgnCJtw4fj9Hx/7cb84edaa9eca04b607f9193162994de/CG000204_ChromiumNextGEMSingleCell3_v3.1_Rev_D.pdf
Deposited data
Single cell-based RNA sequencing data	https://www.ncbi.nlm.nih.gov/geo/	GEO:GSE218201
Experimental models: Organisms/strains
C57BL/6J mice	The Jackson Laboratory
mdx5cv mice	The Jackson Laboratory; Chapman VM, et al. PNAS 86(4): 1292–6.
Oligonucleotides
Gapdh (Forward): 5′-GTGTTCCTACCCCCAATGTGTC-3′	IDT DNA Oligos	N/A
Gapdh (Reverser): 5′-GTAGCCCAAGATGCCCTTCAGT-3′	IDT DNA Oligos	N/A
Col1a1 (Forward): 5′-GCTCCTCTTAGGGGCCACT-3′	IDT DNA Oligos	N/A
Col1a1 (Reverse): 5′-CCACGTCTCACCATTGGGG-3′	IDT DNA Oligos	N/A
Col3a1 (Forwad): 5′-AACCTGGTTTCTTCTCACCCTTC-3′	IDT DNA Oligos	N/A
Col3a1 (Reverse): 5′-ACTCATAGGACTGACCAAGGTGG-3′	IDT DNA Oligos	N/A
Fn1 (Forward): 5′-AAACTTGCATCTGGAGGCAAACCC-3′	IDT DNA Oligos	N/A
Fn1 (Reverse): 5′-AGCTCTGATCAGCATGGACCACTT-3′	IDT DNA Oligos	N/A
Igf1 (Forward): 5′-CTACAAAAGCAGCCCGCTCT-3′	IDT DNA Oligos	N/A
Igf1 (Reverse): 5′-CTTCTGAGTCTTGGGCATGTCA-3′	IDT DNA Oligos	N/A
Ccn2 (Forward): 5′-AGGACTGCAGCGCGCAATGT-3′	IDT DNA Oligos	N/A
Ccn2 (Reverse): 5′-GAGGCCCTTGTGTGGGTCGC-3′	IDT DNA Oligos	N/A
Mmp2 (Forward): 5′-GCCCCGAGACCGCTATGTCCACT-3′	IDT DNA Oligos	N/A
Mmp2 (Revers): 5′-GCCCCACTTCCGGTCATCATCGTA-3′	IDT DNA Oligos	N/A
Timp1 (Forward): 5′-ACTCGGACCTGGTCATAAGGGC-3′	IDT DNA Oligos	N/A
Timp1 (Reveres): 5′-TTCCGTGGCAGGCAAGCAAAGT-3′	IDT DNA Oligos	N/A
Timp2 (Forward): 5′-GGCAACCCCATCAAGAGGA-3′	IDT DNA Oligos	N/A
Timp2 (Reverse): 5′-CCTTCTGCCTTTCCTGCAATTAG-3′	IDT DNA Oligos	N/A
Mmp14 (Forward): 5′-CTTCAAAGGAGATAAGCACTG-3′	Millipore Sigma	KiCqStart® SYBR® Green Primers: Cat# KSPQ12012
Mmp14 (Reverse): 5′-AGAAGTAGGTCTTCCCATTG-3′	Millipore Sigma	KiCqStart® SYBR® Green Primers: Cat# KSPQ12012
Ltbp4 (Forward): 5′- CCCATTCTTCGAAATATCACC-3′	Millipore Sigma	KiCqStart® SYBR® Green Primers: Cat# KSPQ12012
Ltbp4 (Reverse): 5′- GAAAACCCTCTGAACCATAAG-3′	Millipore Sigma	KiCqStart® SYBR® Green Primers: Cat# KSPQ12012
Lox (Forward): 5′-CACCGTATTAGAAAGAAGCC-3′	Millipore Sigma	KiCqStart® SYBR® Green Primers: Cat# KSPQ12012
Lox (Reverse): 5′-GTCCTTCCTACTTAAGCTAATC-3′	Millipore Sigma	KiCqStart® SYBR® Green Primers: Cat# KSPQ12012
Loxl1 (Forward): 5′-TGCTATGACACATACAATGC-3′	Millipore Sigma	KiCqStart® SYBR® Green Primers: Cat# KSPQ12012
Loxl1 (Reverse): 5′-GAACAATGTACTTGGGGTTC-3′	Millipore Sigma	KiCqStart® SYBR® Green Primers: Cat# KSPQ12012
Software and algorithms
GraphPad Prism 8	GraphPad	https://www.graphpad.com/scientific-software/prism/
FlowJo V10	BD Biosciences	https://www.bdbiosciences.com/en-us/products/software/flowjo-v10-software
Image Studio software	LI-COR Biosciences	https://www.licor.com/bio/image-studio/
Image J	NIH	https://imagej.nih.gov/ij/
R package Seurat version 4.0	Satija Lab at NYGC	https://satijalab.org/seurat/
g:Profiler		https://biit.cs.ut.ee/gprofiler/gost
Monocle3		https://cole-trapnell-lab.github.io/monocle3/