FIG. 3.

Eosinophils in BAL fluid of C57BL/6, IL-4^−/−, and IFN-γ^−/− mice. C57BL/6, IL-4^−/−, and IFN-γ^−/− mice were immunized and injected i.v. with 200,000 live Mf as described above. One week later, mice were sacrificed, lungs were lavaged, and the total number of eosinophils per milliliter of lavage fluid was determined as described in Materials and Methods. Data are means ± standard deviations for five mice per group. Significantly more eosinophils were recovered from IFN-γ^−/− mice than from C57BL/6 or IL-4^−/− mice (P < 0.05). This experiment was repeated twice with similar results.