FIG. 4.

Cytokine responses in splenocytes from C57BL/6, IL-4^−/−, and IFN-γ^−/− mice. Splenocytes were isolated from C57BL/6, IL-4^−/−, and IFN-γ^−/− mice after immunization and injection with B. malayi Mf as described above and were stimulated in vitro with 2 μg of anti-CD3/ml. Culture supernatants were recovered 72 h later, and IFN-γ, IL-4, and IL-5 levels were measured by ELISA. Results are means ± standard deviations for five mice per group, and data are representative of three experiments. P < 0.05 for differences in IFN-γ production between C57BL/6 and IL-4^−/− mice and for differences in IL-4 production between C57BL/6 and IFN-γ^−/− mice.