Fig. 7.

NCOR1 expression and T cell polarization in human PBMCs derived moDCs

A. Scatter plot showing the correlation between NCOR1 and tolerogenic genes IL-10 and SOCS3 from a publicly available RNAseq dataset. (n = 5)

B. Violin plot showing the normalized counts of NCOR1 in human conventional DCs in immature, mature, and in different tolerogenic setup (Dex, VD3 and rapamycin treated groups), from publicly available RNAseq datasets. (n = 4)

C. Schematic showing the experimental outline used for differentiation of mature and tolerogenic (Dex-treated) moDCs to check the mRNA (RT-qPCR) and protein level expression of NCOR1.

D. Bar plots depicting the relative mRNA expression of NCOR1, IL-10, IL-12, HK-2 and LDHA in mature and tolerogenic moDCs after 24h pIC stimulation. (n = 4–5)

E. Representative microscopic images showing the NCOR1 protein levels (red) in mature and tolerogenic moDCs after 24h pIC stimulation. Corresponding bar graph depicted the quantification of the NCOR1 intensities in the microscopic images. (n = 5)

F. Schematic showing the CD4 T cell proliferation and T cell subtype profiling after 24h pIC stimulation in the co-culture of CD4⁺ T cells with mature and tolerogenic moDCs with and without KC7F2 and etomoxir treatment.

G. Representative FACS contour plots showing the gating of P1 and P2 populations of eF670 stained CD3⁺CD4⁺ T cells upon co-culture with mature and tolerogenic moDCs with and without combined treatment with KC7F2 and etomoxir after 72h after 24h pIC stimulation. Corresponding bar graph depicted the cumulative MFI shifts calculated from independent biological replicates and histogram showed the representative MFI shifts observed. (n = 5)

H. Representative FACS contour plot showing the gating of IFN-γ⁺ and T-bet⁺ populations gated on CD3⁺CD4⁺T cells upon co-culturing with moDCs for 96 h. Scatter plots and bar graphs depicted the cumulative percent positive and MFI shifts of IFN-γ⁺ and T-bet⁺ cells in mature and tolerogenic moDCs conditions with and without combined treatment with KC7F2 and etomoxir at 24h pIC stimulation.(n = 5)

*p ≤ 0.05, **p ≤ 0.01, ***p ≤0.001 and ****p ≤0.0001. p-value has been calculated using two tailed unpaired student’s t-test. Error bars represent SEM. . (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)