Figure 3.

E2F7 depletion in Hep3B cells suppresses tumor invasion and metastasis in an orthotopic xenograft mouse model. Mouse liver orthotopic transplantation model was constructed through 4–5‐week‐old male BALB/c nude mice. (A) The orthotopic tumor growth was significantly suppressed in the mice treated with E2F7 depleted Hep3B cells. (B) Depletion of E2F7 in Hep3B cells led to a significant decrease in the xenograft tumor weight (**p < 0.01). (C) The xenograft tumor specimens were collected and checked under HE staining examination. Depletion of E2F7 in Hep3B cells induced fewer intrahepatic metastasis lesions in the mouse models than in the control ones (**p < 0.01). (D)Depletion of E2F7 in Hep3B cells resulted in fewer metastasis lesions in the lung of the mouse models than in the control (**p < 0.01). HE, hematoxylin and eosin. [Color figure can be viewed at wileyonlinelibrary.com]