FIG. 6.

Transfection of TLR2 confers responsiveness to P. gingivalis LPS in HEK 293 cells. HEK 293 cells were transiently transfected with human TLR2 plus the ELAM-1 luciferase reporter construct. Cells were stimulated for 6 h with increasing doses of repurified P. gingivalis LPS. Supernatants were analyzed for IL-8 secretion, and cell lysates were analyzed for NF-κB nuclear translocation, expressed in luciferase units.