Table 1.
Kinetic and thermodynamic parameters of conformational exchange for the investigated K‐Ras variants in their GDP‐ and GTP‐bound forms deduced from a global fit analysis of 15 N backbone relaxation dispersion NMR measurements (T=25 °C, pH 7.4).
|
|
K‐Ras‐wt |
K‐Ras‐G12C |
K‐Ras‐G12D |
K‐Ras‐G12V |
|---|---|---|---|---|
|
|
GDP‐bound forms |
|||
|
k ex/s−1 |
164±28 |
99±26 |
345±47 |
172±35 |
|
p E/% |
2.8±0.2 |
2.4±0.2 |
1.7±0.2 |
1.5±0.2 |
|
k GE/s−1 |
4.6±0.8 |
2.4±0.7 |
5.9±1.1 |
2.6±0.6 |
|
k EG/s−1 |
159±32 |
97±28 |
339±73 |
169±47 |
|
ΔG GE/kcal/mol |
2.1±0.2 |
2.2±0.2 |
2.4±0.2 |
2.5±0.2 |
|
|
GTP‐bound forms |
|||
|---|---|---|---|---|
|
k ex/s−1 |
404±54 |
500±67 |
332±42 |
312±51 |
|
p E/% |
7.8±0.2 |
4.5±0.3 |
8.4±0.3 |
5.9±0.3 |
|
k GE/s−1 |
32±4 |
23±3 |
28±4 |
18±3 |
|
k EG/s−1 |
373±52 |
478±78 |
304±41 |
294±52 |
|
ΔG GE/kcal/mol |
1.5±0.1 |
1.8±0.1 |
1.4±0.1 |
1.6±0.1 |