FIG. 1.

 Composite agarose gels stained with ethidium bromide (A and C) and Southern blot (B and D) of PCR and RT-PCR products. PCR was performed with either losB primers (panels A and B) or ftpA primers (panels C and D). Lanes 1 and 2 contain 35000HP genomic DNA and no template, respectively. cDNA (lanes 3, 5, 7, and 9) and RNA that was not reverse transcribed (lanes 4, 6, 8, and 10) were prepared from biopsy 165 (lanes 3 and 4), biopsy 164 (lanes 5 and 6), uninfected skin homogenized with H. ducreyi (lanes 7 and 8), and uninfected skin (lanes 9 and 10).