TABLE 1.

Primers

Primer	Gene	Sequence (5′ to 3′)	Amplicon size (bp)	Limit of detection (CFU)a
18set5′	pal	TTGAGAATTCAGTAGTTCATCAGGTAAAACAGATG	400	101
18set3′		TAAAGAATTCAAATTAGTACTCTAATACTGCACGG
28set5′	hlp	TTGAGAATTCGATAAGCCTGCAAACAACCCTGTAG	578	103
28set3′		CCAAGAATTCGGTTTATTTGTTTTCGTCAGCTTC
Pilin1	ftpA	GACCACACAGTGCCAGGT	220	101–102
PilinR2		GCGTTGCCATACGTTCAGC
Momp-for-2	momp	CTGCACCAGATGCCAATACC	700	103
Momp-rev-2		TGATAACCCTACAGAAACAGAATG
OmpA2-for-5	ompA2	TGGATTAGCGCGTAACGATTATAGTG	315	101–102
OmpA2-rev-5		GCTCGTCCATCATCATTTGCG
TdhA-for-2	tdhA	TGATAAAGGCAACCACTACCCAC	550	102
TdhA-rev-2		CTGCGCGACAGTATTAATCCAAGG
HgbA-for-2	hgbA	GCTCAGGTTATGCTATTCGTGGTGT	549	101–102
HgbA-rev-2		CATAGCCTAGGGTAAAGCGGTTGGT
Lst-for-1	lst	GGTTACGTGGTTTAGATGTTTATGGTGC	423	101–102
Lst-rev-1		CGATATATATCAATCTTTTCTGCTTCTGTC
LosB-for-1	losB	CGTAATAGGGATTTATTGCTTTTGAGAG	513	102–103
LosB-rev-1		CAGCAATATGCACAATAGAAGTATCAGGTG
Cdt3	cdtB	AGGCGGTTCTGATGCGGTAAG	359	102
Cdt4		AGCGATCACGAACAAAACTAACAG
HhdA-for-1	hhdA	GGCGGTCAAAATATAATGGAAAGAG	565	102–103
HhdA-rev-1		TCGTGCCCGGCTTGGATACTAC
P10	lspA1	TTGAATTCAAGTTTCAGCAGGGACAGCAAATATC	264	101–102
P11		TTTCTCGAGTCTAATTTTTCGGCGACAAGG
P12	lspA2	AAGTTTCAGCAAGAGCGGC	320	102–103
P13		TATTGGCTGCAAGCTCTG
β-actin 5′	β-actin	ATCTGGCACCACACCTTCTACAATGAGCTCCG	837	ND
β-actin 3′		CGTCATACTCCTGCTTGCTGATCCACATCTGC

^aValue represents the range of limits of detection for three experiments. ND, not determined.