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. Author manuscript; available in PMC: 2023 Apr 26.
Published in final edited form as: Sci Transl Med. 2022 Oct 26;14(668):eabn5166. doi: 10.1126/scitranslmed.abn5166

Figure 7: Subdoligranulum isolate 7 is detectable in the feces of individuals in the at-risk period and early stages of RA.

Figure 7:

(A) Known quantities (CFU/ml) of Subdoligranulum isolate 7 were spiked into a human fecal sample to create a standard curve. The curve ranges from 1×109 CFU of isolate 7 per 100 mg of feces to 0 CFU of isolate 7, with a limit of detection set at 1×105 CFU per 100 mg of feces. A line of best fit was set for regression analysis (R2=0.9982). (B) Feces from healthy controls (n=12), individuals at-risk for RA (n=12), individuals with early RA (n=12), or SPF mice (n=12) were analyzed by qPCR for presence of isolate 7. The number and percentage of samples above the limit of detection for the assay is displayed by group. ***P<0.001, Chi-square test. (C) Regression analysis was performed utilizing the line of best fit for the assay to determine the estimated CFUs of isolate 7 in the positive samples. The mean CFUs per 100mg feces is displayed ± SEM for all positive samples (n=4), for positive samples from individuals at-risk for RA (n=2), and for positive samples for individuals with early RA (n=2).