FIGURE 2.

Pulmonary ECs facilitate more neutrophil transmigration upon addition of HLA class I antibodies compared to IgG2a treated cells (A) number of transmigrated neutrophils during flow assay after 5 h treatment with PBS or 1 ng/ml LPS and 2 h with IgG2a or HLA‐ABC antibody as indicated. Data points represent neutrophil (PMN) trans‐endothelial migration (TEM) per field of view (FOV). Three fields of view per channel were obtained during time‐lapse imaging, followed by tile scans with 25 additional fields of view per condition and replicate at endpoint to determine the distribution of neutrophils. Bars represent average TEM of PMN/FOV for all experiments. Two‐way ANOVA was used to determine statistical significance. **** = p < .0001, error bars are presented as standard error of mean (SEM), n = 3. (b) Representative images from time‐lapse videos as indicated. White arrows indicate neutrophils (individual or clusters), white star indicates EC disturbance