FIGURE 4.

Effects of sequential treatment with (d‐Arg³⁵)‐sea lamprey (SL)‐PYY and liraglutide on peripheral insulin sensitivity and pancreatic hormone content in HFF‐STZ mice. (A–D) Variables were assessed after 28 days of twice‐daily (08:00 AM and 08:00 PM) treatment with liraglutide alone (0.25 nmol/kg bw) or SL‐PYY (75 nmol/kg bw) at 08:00 AM and liraglutide (0.25 nmol/kg bw) at 08:00 PM. (A) Blood glucose was assessed prior to and after administration of insulin (15 U/kg bw, i.p.) at t = 0 min. (B) Associated 0‐90 minute glucose area above the curve (AAC) values are also shown. (C) and (D) Pancreatic hormone contents were assessed on day 28 following acid/ethanol protein extraction. Values are mean ± SEM for n = 8 mice. *P < .05 and ***P < .001 compared with saline‐treated HFF‐STZ control mice. ^Δ P < .05 compared with twice‐daily liraglutide‐treated HFF‐STZ mice. HFF, high fat fed; i.p., intraperitoneal; PYY, peptide tyrosine tyrosine; STZ, streptozotocin