Figure 1.

MiR‐27a affects the expression of 14‐3‐3θ and RUNX1 in t(4;11) leukemia cells. (A) MiR‐27a and 14‐3‐3θ transcript levels in different leukemia cell lines; PBLs are used as reference. Relative gene expression was determined by the $2^{-\mathrm{\Delta \Delta }{C}_{\mathrm{t}}}$ method. (B) MiR‐27a and 14‐3‐3θ transcript level in t(4;11) versus t(12;21) ALL patients. Relative gene expression was determined by the $2^{-\mathrm{\Delta \Delta }{C}_{\mathrm{q}}}$ method, without the control sample. Western blot analysis of (C) 14‐3‐3θ and (D) RUNX1 protein level in RS4;11 cells transfected with MiR‐27a or MiR‐negative control; αTubulin, loading control. Error bars indicate standard deviations of triplicate experiments. MiR, microRNA; NS, not statistically significant; PBL, peripheral blood leukocytes; R.U., relative units; WCE, whole‐cell extract. *p< .05; **p< .01; ***p < .005.