Skip to main content
. 2022 Aug 18;40(7):706–717. doi: 10.1002/cbf.3736

Figure 2.

Figure 2

AF4 and MLL‐AF4 are novel targets of MiR‐27a. (A) Western blot analysis of AF4 and MLL‐AF4 levels in RS4;11 cells transfected with MiR‐27a and with MiR‐negative control; αTubulin, loading control (upper panel). Western blot analysis of AF4 and MLL‐AF4 levels in RS4;11 cells transfected with anti‐MiR‐27a and the anti‐MiR‐negative control; vinculin, loading control (lower panel). (B) AFF1 transcript levels were measured 24 h after cell transfection with MiR‐27a or MiR negative control. Relative gene expression was determined by the 2−ΔΔCt method. (C) Sequence alignment of MiR‐27a and its putative seed within AFF1‐3′‐UTR, and dual‐luciferase reporter assay results in RS4;11 cells cotransfected with MiR‐27a and the psiCHECK‐2 vector containing AFF1‐3′‐UTR WT or MUT, and the relative controls. In bold are the nucleotides mutated in the MUT construct. Error bars indicate standard deviations. miR, microRNA; MLL, mixed‐lineage leukemia; MUT, mutant; R.U., relative units; UTR, untranslated region; WCE, whole cell extract; WT, wild type. *p< .05; **p< .01.