Figure 3.

MiR‐27a impairs the expression of MLL‐AF4 target genes in t(4;11) cell lines. (A) HOXA9, HOXA7, and MEIS1 transcript expression in RS4;11, SEM, MV4‐11, and REH, 24 h after transfection with MiR‐27a or MiR‐negative control. Relative gene expression was determined by the $2^{-\mathrm{\Delta \Delta }{C}_{\mathrm{t}}}$ method. (B) Interaction of MLL‐AF4 and wild‐type MLL with HOXA9 gene promoter (HOXA9 pr) after RS4;11 cell transfection with MiR‐27a or MiR negative control, determined by ChIP. ACTB promoter (β‐actin pr) is the negative control. Data are expressed as a percentage of HOXA9 and ACTB promoters in immunoprecipitated chromatin with respect to the INPUT. IgG is the mock (left panel). Error bars indicate standard deviations. ChIP, chromatin immunoprecipitation; MLL, mixed‐lineage leukemia; NS, not statistically significant; R.U., relative units. *p< .05; **p< .01, ***p < .005.