Triple pathological staining of autopsy cases of different studies in PSP‐RS and AD. The three columns on the left show the pathological sections of the GP, which was the most important region for the discrimination of PSP‐RS, and that were stained with PM‐PBB3, Gallyas‐Braak, and AT8 antibodies. The top three rows show the PSP‐RS, HC, and AD groups, with PSP‐RS presented from the lowest to highest PSP rating scale. The three right columns show the pathological sections of the amygdala, which was the most important region for the discrimination of AD, triple‐stained in the same way. The images show that tau was well captured by the three stains in the GP of PSP‐RS cases and the amygdala in AD cases. PM‐PBB3 staining shows green for fluorescence and yellow for autofluorescence. The orange arrowhead indicates a typical tau lesion consistent with triple staining, whereas the white arrowhead indicates typical autofluorescence. AD, Alzheimer's disease; GP, globus pallidus; HC, healthy control; PSP‐RS, progressive supranuclear palsy–Richardson's syndrome. [Color figure can be viewed at wileyonlinelibrary.com]