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. 2022 Aug 2;179(21):4958–4973. doi: 10.1111/bph.15921

FIGURE 1.

FIGURE 1

Integrin α4β1 and α9β1 bind to SVEP1 immunoblots with anti‐α4 (a) and anti‐GFP (b) antibodies following immunoprecipitation of protein lysates using anti‐FLAG agarose from HEK293A or HEK293A cells stably overexpressing SVEP1‐FLAG transfected with integrin α4β1 (α4) or HEK293A cells co‐transfected with GFP only control, or GFP‐integrin α9β1 (α9) and SVEP1‐FLAG plasmids. Binding efficiency of HEK293A cells stably overexpressing integrin α4β1 (c) or α9β1 (d) to surface coated with 100 nM mannose binding protein (MBP), or SVEP1 21st or 22nd CCP domain (±SD, n = 3). Data are normalised to MBP control to account for variation in cell binding between experiments.