FIGURE 6.

Integrin α4/9 regulates blood vessel contraction via Ca²⁺ influx through VGCCs in a PKC and ROCK dependent manner (a) aortas from C57BL/6J mice were incubated with the dual integrin α4/9 inhibitor BOP overnight and incubated with the VGCC blocker nifedipine (NF) for 30 min prior to U46619 application and force generation was recorded; NS, non‐stimulated control. Data presented are means, with 95% confidence intervals; n = 7. * P < 0.05, NS significantly different from NF; # P < 0.05, BOP significantly different from BOP + NF; mixed effect models. (b) Aortas from Svep1 ^+/− mice were incubated with NF. Data presented are means, with 95% confidence intervals; n = 6. *P < 0.05, significant effect of NF; mixed effect models. (c) Aortas from C57BL/6J mice were incubated with BOP overnight and incubated with the PKC inhibitor BIM (I) for 30 min prior to U46619 application; NS, non‐stimulated control. Data presented are means, with 95% confidence intervals; n = 7. * P < 0.05, NS significantly different from BIM (I), # P < 0.05, BOP significantly different from BOP+BIM (I); mixed effect models. (d) Aortas from Svep1 ^+/− mice were incubated with BIM (I). Data presented are means, with 95% confidence intervals;.n = 9. *P < 0.05, significant effect of BIM (I); mixed effect models. (e) Aortas from Svep1 ^+/− mice (+/−) or littermate control mice (+/+) were incubated with the ROCK inhibitor Y27632 for 30 min prior to U46619 application and force generation was recorded. Data presented are means, with 95% confidence intervals; n = 7. *P < 0.05, significant effect of Y2673 in control mice, # P < 0.05, significant effect of Y2673 in Svep1 ^+/− mice; mixed effect models.