Fig. 5.

Effect of TIMP-2 suppression on proliferation. The cell lines/cells studied have been described in Fig. 3. A, C Proliferation evaluated by Ki67 staining. Immunofluorescence of Ki67 (a marker of proliferation) staining was performed as described in “Methods”. Representative images of Ki67 positive cells (red) and DAPI (blue) combined. ×20 magnification; scale bar (in white) 20 µM. B, D Proliferation evaluated by EdU staining. Cells were stained for EdU and analysed by flowcytometry as described in “Methods”. Results are expressed as % of EdU positive cells (or cells in the S-phase of cell cycle progression). C, E, F mRNA expression of Ki67, CDC25A, CDC25B and CDC25C were evaluated by qRT-PCR. Bar graphs are representative of three independent experiments in triplicate. Significance was obtained using One-way ANOVA and is indicated by *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001