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Temperature dependence of cytosine NH2 rotations in the 15-bp DNA duplex. (A) Rate constants (krot) for cytosine NH2 rotations measured at 15, 20, 25, and 30°C. (B) Energy barriers for cytosine NH2 rotations. The activation free energy and its enthalpic and entropic components were determined from the temperature dependence of krot constants.
