TABLE 3.

Characterization of bvMSP1₄₂

bvMSP142	% Final puritya	Endotoxin level (U/mg)b	Mass (kDa) determined by:		N-terminal sequenced	Glycosylation pattern	Monoclonal antibody recognitione
			Gel filtration chromatography	Electron spray mass spectroscopyc
Secreted	97.4	0.78	81.5f	43,320.0; 44,404.0f	NH2 ADPAVTPSVIDNIL	High mannose	5.2, 2D634, 13E353, 4H919, 12.1, 1E1, 12.8, 2F10
Intracellular	ND	ND	100.0	41,320; 42,276; 43,660	NH2 ADPAVTPSVIDNIL	High mannose	Same as for secreted bvMSP142

^aDetermined by scanning densitometry. ND, not determined. 

^bDetermined by Limulus amebocyte lysate assay. 

^cMajor mass peaks are listed. 

^dADP is a vector-derived cloning sequence; underlining indicates the start of the MSP1₄₂ sequence. The predicted sequence is _NH2 ADPAVT. 

^eMonoclonal antibodies recognizing conformational epitopes in MSP1₁₉ with different specificities were used in Western blot analyses: 5.2 (32), 2D6₃₄, 13E3₅₃, 4H9₁₉ (7), 12.1 (25), 1E1, 12.8, and 2F10 (16). Antibodies 5.2 and 12.8 recognize the first EGF domain; 4H9₁₉ recognizes the second EGF domain; and 2D6₃₄, 13E3₅₃, 12.1, and 12.8 recognize both EGF domains. 

^fPredicted: 41,924.4.