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. Author manuscript; available in PMC: 2022 Dec 31.
Published in final edited form as: Mater Sci Eng C Mater Biol Appl. 2015 Dec 30;61:437–448. doi: 10.1016/j.msec.2015.12.090

Fig.5.

Fig.5.

Effects of culturing on ECM on catalase and GPx1 in UC-MSCs. (A) Culturing on ECM upregulated CAT mRNA expression in UC-MSCs, determined by real-time RT-PCR. (B) The protein levels of catalase, measured by western blot analysis, did not change in cells cultured on TCPS and ECM. (C) Culturing on ECM improved catalase activity in UC-MSCs. (D) The mRNA level of GPX1 did not change in ECM-cultured cells. (E) The protein level of GPx1 did not change in ECM-cultured cells. Values are the mean ± S.E. of four independent experiments (n = 4) in real-time RT-PCR, western blot analysis, and catalase activity analysis. Statistically significant differences are indicated by * (p < 0.05) or ** (p < 0.01). P: TCPS/ tissue culture polystyrene; E: ECM/extracellular matrix.