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. Author manuscript; available in PMC: 2022 Dec 31.
Published in final edited form as: J Tissue Eng Regen Med. 2017 Jun 20;12(2):e1008–e1021. doi: 10.1002/term.2422

Figure 3.

Figure 3.

The involvement of the SIRT1/p38/p16INK4α pathway in DECM-mediated anti-senescence. UC-MSCs were pretreated with 100 μM H2O2 for 2 h to induce premature senescence. The mRNA levels of P16INK4A (A) and SIRT1 (B) were measured in quadruplicate samples by real-time RT-PCR. Values are the mean ± S.E. of four independent experiments (n = 4). Western blotting was used to measure the protein levels of p16INK4α (C), SIRT1 (D), p53 (E), and p21 (F) in triplicate samples. (G) The activation of the p38 signaling pathway was evaluated. The levels of p-p38 were normalized to total p38 protein. The levels of p38 were normalized to α-tubulin protein. Values are the mean ± S.E. of three independent experiments (n = 3). Statistically significant differences are indicated by * (p < 0.05).