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. Author manuscript; available in PMC: 2022 Dec 31.
Published in final edited form as: Mater Sci Eng C Mater Biol Appl. 2019 Oct 7;106:110286. doi: 10.1016/j.msec.2019.110286

Fig. 2.

Fig. 2.

The effect of DECM expansion on the redifferentiation of chondrocytes. TCPS- or DECM-expanded chondrocytes were induced to redifferentiation in a pellet culture system for 21 days. (A) Safranin O (S.O.) was used to stain sulfated GAGs and immunohistochemistry staining (IHC) was used to detect COL II. Scale bar = 500 μm. (B-D) RT-qPCR was used to measure the mRNA levels of chondrogenic marker genes, COL2A1 (B), ACAN (C), and SOX9 (D) between TCPS- and DECM-expanded chondrocytes. Values are presented as the mean ± S.E.M. of four independent experiments (n = 4) in RT-qPCR experiments. Statistically significant differences are indicated by * p < 0.05 or ** p < 0.01 between the indicated groups.