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. Author manuscript; available in PMC: 2022 Dec 31.
Published in final edited form as: Mater Sci Eng C Mater Biol Appl. 2019 Oct 7;106:110286. doi: 10.1016/j.msec.2019.110286

Fig. 7.

Fig. 7.

DECM expansion enhanced the anti-inflammatory properties of chondrocytes through the up-regulation of SIRT1. TCPS- or DECM-expanded chondrocytes were induced to redifferentiation in the presence of IL-1β (1 ng/mL) or TNF-α (1 ng/mL). (A) The mRNA levels of SIRT1 in chondrocytes were quantified using RT-qPCR. Values are presented as the mean ± S.E.M. of four independent experiments (n = 4) in RT-qPCR experiments. (B) The protein levels of SIRT1 and SOX9 in chondrocytes were determined using Western blot assays. (C) Quantification of SIRT1 protein levels in TCPS- and DECM-expanded chondrocytes. (D) Quantification of SOX9 protein levels in TCPS- and DECM-expanded chondrocytes. Values are presented as the mean ± S.E.M. of three independent experiments (n = 3) in Western blot assays. Statistically significant differences are indicated by * p < 0.05 or ** p < 0.01 between the indicated groups; # p < 0.05 or ## p < 0.01 versus TCPS-expanded untreated cells; Ψ p < 0.05 or ΨΨ p < 0.01 versus DECM-expanded untreated cells.