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. Author manuscript; available in PMC: 2022 Dec 31.
Published in final edited form as: Mater Sci Eng C Mater Biol Appl. 2019 Oct 7;106:110286. doi: 10.1016/j.msec.2019.110286

Fig. 8.

Fig. 8.

Inhibition of SIRT1 abrogated the DECM-enhanced anti-inflammatory properties of chondrocytes. DECM-expanded chondrocytes were induced to redifferentiation and treated with 10 mM of NAM in the presence of 1 ng/mL of IL-1β and TNF-α for 21 days. (A) Safranin O (S.O.) was used to stain sulfated GAGs and immunohistochemistry staining (IHC) was used to detect COL II. Scale bar = 500 μm. (B-E) The mRNA levels of COL2A1 (B), ACAN (C), SOX9 (D), and SIRT1 (E) were evaluated by RT-qPCR. Values are presented as the mean ± S.E.M. of four independent experiments (n = 4) in RT-qPCR experiments. (F) The protein levels of SIRT1 and SOX9 in chondrocytes were determined using Western blot assays. Statistically significant differences are indicated by * p < 0.05 or ** p < 0.01 between the indicated groups.