FIG. 4.

Anti-TTFC IgA levels in BALF from groups of eight mice immunized intranasally with a single dose (sd) or double dose (dd) of nonexpressing recombinant L. plantarum 8826(pTG2247) or TTFC-producing L. plantarum 8826 (pMEC4) (constitutive promoter, low TTFC dose) or 8826(pMEC46) (inducible promoter, high TTFC dose) or with buffer alone (PBS) as a control. Individual BALF samples were collected 10 days after the last boost, and specific IgA response was normalized according to the level of total IgA and expressed in specific activity (SA). Bars represent the mean IgA level ± SEM in each group.