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. 2022 Dec 31;12:22628. doi: 10.1038/s41598-022-27199-3

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of SameOrco, SameOR1, and SameOR2 in antenna and palp tissue sections, respectively. RNAscope in situ hybridization was used to characterize the spatial distribution of these putative ORs. (a–c) Probes against SameOrco (green) in antenna sections (a, b) and palp sections (c). Orco⁺ cells in trichoid (Tr) and basiconic (Ba) sensilla are highlighted by arrows in panel a. Trichoid sensilla containing one, two, and three ORNs are depicted by arrows in panel (b). (d, e) SameOrco probe with the probe against the second most expressed putative SameOR in each tissue type were used. SameOR1 (antenna, panel d) and SameOR2 (palp, panel e) are present only in Orco⁺ cells. Blue label in all images:DAPI. Scale bars: 10 μm in panels a–c; 15 μm in panels d–e.