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. 2022 Dec 31;12:22628. doi: 10.1038/s41598-022-27199-3

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of SameOR71, SameOR58, and SameOR160 expression in trichoid sensilla. RNAscope in situ hybridization was used to determine the spatial distribution of these putative ORs. (a) Examples of antenna sections showing different combinations of probes against SameOrco and SameOR58 + SameOR160 (top), SameOR58 + SameOR71 (middle), and SameOR160 + SameOR71 (bottom), respectively. (b) Examples of individual trichoid sensilla containing one, two, or three ORNs and different combinations of the SameORs tested and indicated by gray arrows. Note that the images showing one and two ORNs are the same as in Fig. 3b but with additional color channels. Middle panel: red arrows: a cluster of three Orco⁺ cells that do not express either of the two tested putative SameORs. (c) Summary of the configurations observed in trichoid sensilla. Scale bars: 20 and 10 μm in panel a and b, respectively.