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SDS-PAGE analysis of purified recombinant TbpA and TbpB. Both proteins were analyzed using denaturing (5 min at 100°C, in loading gel with β-mercaptoethanol) and nondenaturing (no boiling, no β-mercaptoethanol) conditions. The sensitivity of the tertiary structure of TbpA is demonstrated by the change in banding pattern on boiling. The tertiary structure of TbpB withstands this treatment, as evidenced by the similarity of the banding pattern before and after boiling.
