FIG. 2.

Western blot analysis of recombinant Tbps. Lanes: 1, molecular mass markers (sizes as indicated); 2, rTbpA probed with anti-native meningococcal TbpA antisera; 3, rTbpB probed with anti-native meningococcal TbpB antisera; 4, rTbpB probed with hTf-HRP. Certain epitopes on TbpA must retain their native conformation; hence, TbpA and its degradation products are detected. The abundance of truncated TbpB derivatives is evident. All of these are recognized by antibodies to native TbpB (lane 3), and most are functional, as demonstrated by probing with hTf-HRP (lane 4).